| 1. | Prediction of subcellular localization of proteins for gram - negative bacteria
革兰氏阴性菌中蛋白质亚细胞定位预测 |
| 2. | For further identification the existence of the extracellular ecbp21 , we used immunogold localization technology to study the subcellular localization of ecbp21
为了进一步确定ecbp21的胞外存在，本实验利用免疫金标定位技术，研究了ecbp21亚细胞定位状况。 |
| 3. | Subcellular localization by immunofluorescence revealed that two rat isoforms are associated with the autophagic pathway . these results are help to discover the mechanisms and functions of autophagy in vivo
另外，通过荧光定位以及与mdc的共定位分析发现两个基因与大鼠的lc3相似，也是定位在自噬体上。 |
| 4. | Studies on the subcellular localization of gfp - trpt1 / pit13 showed the trpt1 and pit 13 proteins localized predominantly to nuclear bodies . this is consistent with where the trpt1 functions
Trpti与gfp融合蛋白在细胞内的表达结果，显示trpti主要分布在细胞核内，这与其具有的参与trna剪接成熟的功能是相符合的。 |
| 5. | To study the biological function of sh2a gene , we constructed its recombinant expression vec - tor , and investigated its function by cell transfection , kinase assay , subcellular localization and expression analysis . materials and methods 1
我们构建了sh _ 2a基因的真核重组表达载体，通过细胞转染、激酶活性检测、亚细胞定位、表达分析和流式细胞仪等方法对其功能进行了初步的研究。 |
| 6. | In order to understand the apoplast cam gene and the relationship between the apoplast cam and cam isoforms , we intended to get transgenic plants harboring soybean calmodulin isoform genes ( scams ) fused to gfp as a reporter and study the subcellular localization of scams
为了进一步利用分子生物学方法在基因水平上研究胞外cam的存在，同时研究胞外cam与cam亚型之间的关系，本论文利用绿色荧光蛋白( gfp )作为报告基因研究了大豆cam基因家族( scams )的亚细胞定位。 |
| 7. | The cellular localization of hse was done by using in situ hybridization , the results showed that primary spermtatocytes and spermatids in seminiferous tubular have positive signals . the subcellular localizations of hsei and hseii were identified by gfp fusion protein . hsei - gfp fusion protein was evenly distributing in cytoplasm , while hseii - gfp was not evenly distributing in cytoplasm and there were many bright spots in cytosol
用gfp高合蛋白的技术确定hsei和hsell在细胞中勺定位，结果显示hsei gfp均匀地分布于细胞浆中，而hsell0fp则分布不均匀，在胞浆有聚集成许多荧光信号很强的点。 |
| 8. | An unusual rice calmodulin isoform , oscam61 , was first obtained in our lab , which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site . in vitro activity assays confirm oscam61 as a functional calmodulin . using the green fluorescent protein ( gfp ) as a visual marker , we further studied subcellular localization of oscam61 in stably transformed tobacco cells
利用绿色荧光蛋白( greenfluorescentprotein , gfp )作为标记，研究了oscam61在烟草细胞中的定位, gfp - oscam61融合蛋白（具有开放的异戊烯化修饰位点）定位于细胞质膜和细胞器膜上，而oscam61 - gfp （异戊烯化修饰位点被gfp封闭）定位于细胞核的核质中。 |
| 9. | Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root
首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。 |
| 10. | In our experiment , after light and dark adaptation , the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution , physiological solution and low calcium solution . we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology . our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而钙离子对gq蛋白亚基活性有无影响还未见报道。我们以光适应和暗适应条件下的罗氏沼虾复眼视网膜为材料，分别用高钙溶液、生理溶液、低钙溶液孵育后，通过sds ? page电泳技术及免疫胶体金电镜技术，研究钙离子浓度对光暗适应时罗氏沼虾感光细胞gq蛋白亚基含量的影响及亚基亚细胞定位的影响。 |
